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Research Projects - Biomolecular Physics Laboratory
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Structure Based Thermodynamic Studies of Proteins
Ongoing and Recently Completed Projects 

 

Fuctional and Biophysical Characterization of PLC-zeta, the “Sperm Factor”
(2009-present)
 Collaborators
 
 
Cardiff University School of Medicine, UK
Professor Anthony Lai  
Wales Heart Research Institute: Cell Signaling Laboratory
Professor Karl Swann
Wales Heart Research Institute: Cell Signaling Laboratory
 Funding
 
 
 Welcome Trust and NCSR "Demokritos"

Publications

› M. Nomikos et al., “Phospholipase Cζ rescues failed oocyte activation in a prototype of male factor infertility", Fertil. Steril. 99, 76 (2013) [Link] 

2013 Award, American Society of Reproductive Medicine (www.fertstert.org)

› M. Theodoridou et al., “Chimeras of sperm PLCζ with PLCδ1 reveal discrete domain roles in the generation of Ca2+ oscillations at fertilization in mouse eggs”, Mol. Hum. Reprod., in press, published on line - doi: 10.1093/molehr/gat070 (2013) [Link] 

› Y. Yu et al., “PLCζ causes Ca2+ oscillations in mouse eggs by targeting intracellular and not plasma membrane PI(4,5)P2”, Mol. Biol. Cell, 23, 371 (2012) [Link]
2013 Award, American Society of Reproductive Medicine (www.fertstert.org)

› M. Nomikos et al., “Phospholipase Cζ binding to Ptdlns(4,5)PIP2 requires the XY-linker region”, J. Cell Sci., 124, 2582 (2011) [Link]

› M. Nomikos et al., “Male infertility-linked point mutation disrupts the Ca2+ oscillation-inducing and PIP2 hydrolysis activity of sperm PLCζ”, Biochem. J., accelerated publication 434, 211 (2011) [Link]
Paper Selected for cover and BJ Central Spotlight, February - March 2011

› M. Nomikos et al., “Novel regulation of PLCζ activity via its XY-linker”, Biochem. J., accelerated publication 438, 427 (2011) [Link]
Paper Selected for cover and BJ Central Spotlight, September 2011 

Protein - Polymer Interactions 
(2010-present)
 Collaborators
 
  
Dr. Dimitris Tsiourvas
IAMPPNM, NCSR "Demokritos"
Dr. Zili Sideratou 
IAMPPNM, NCSR "Demokritos"
Dr. Stergios Pispas 
Institute of Theoretical and Physical Chemistry, NHRF
 Funding
 		  
 NCSR "Demokritos" and NHRF

Publications

› D. Tsiourvas et al., “Guanidinium End-Functionalized PEGylated Oligo(L-Lysine) Derivatives for the Stabilization and Controlled Release of Insulin”, J. Colloid Interface Sci., 384, 61 (2012) [Link] 

› M. Karayianni et al., “Complexation of Lysozyme with Sodium(Sulfamate-Carboxylate) Isoprene Polyelectrolyte”, Biomacromolecules, 12, 1697 (2011)  [Link]

› Z. Sideratou et al., “Guanidinylated poly(L-lysine) dendrigrafts for the stabilization and controlled release of insulin”, J Colloid Interface Sci., 351, 433 (2010) [Link]

The Thermodynamic Stability of TIM-Barrel Functional Domains - Chitinases
(2005-present)
 Collaborators
 
  
Department of Biology, University of Athens
Professor Contantinos E. Vorgias
Laboratory of Protein Structure and Function
 Funding
 		    PENED Program 2003 - NCSR “Demokritos”

Publications

› A.C. Zees et al., “Insights into the role of the (alpha+beta) insertion in the TIM-barrel catalytic domain, regarding the stability and the enzymatic activity of chitinase A from Serratia marcescens”, Biochim. Biophys. Acta, 1794, 23 (2009) [Link] 

› S. Pyrpassopoulos et al., “Equilibrium heat-induced denaturation of Chitinase 40 from Streptomyces Thermoviolaceus”, Proteins, 64, 513 (2006) [Link]
 

The Stability of Pressure-treated Proteins: Applications in the Food Industry
(2011-present)
 Collaborators
 
  
Department of Biology, University of Athens
Professor Petros Taoukis
Department of Synthesis and Development of Industrial Processes
 Funding
 		    NTUA - NCSR “Demokritos”

Publications

› Z. Alexandrakis et al., “Comparative structural changes and inactivation kinetics of pectin methylesterases from two orange varieties subjected to high pressure processing”, Food and Bioprocess Techn., in press, published on-line: 10.1007/s11947-013-1087-7 (2013) [Link]

Biophysical Studies of the BRCT Domains of Human BRCA1, BARD1 and 53BP1 proteins
(2004-2011)
 Collaborators
 
  
Department of Biology, University of Athens
Professor Contantinos E. Vorgias
Laboratory of Protein Structure and Function
 Funding
 		    PEND Program, GSRT 2005 – Empirikion Award – Excellence in Research Program/I-RRP - NCSR “Demokritos”

Publications

› Dimitris Mastellos and George Nounesis, “The BRCT signaling “landscape”: Emerging concepts and implications in tumorigenesis and cancer diagnostics”, Chapter 6, pp 173 – 208, in Cancer Biomarkers, A. Georgakilas Editor, CRC Press, Boca Raton, (2013) [Link] 

› A. Thanassoulas et al., “Thermal and chemical denaturation of the BRCT functional module of human 53BP1”, Int. J. Biol. Macromol., 49, 297 (2011) [Link] 

› A. Thanassoulas et al., “Thermodynamic study of the BRCT domain of BARD1 and its interaction with the –pSer-X-X-Phe- motif-containing BRP1 peptide”, Biochim. Biophys. Acta, 1804, 1908 (2010) [Link]

› S. Pyrpassopoulos et al., “Thermal denaturation of the BRCT tandem repeat region of the human tumour suppressor gene product BRCA1”, Biophys. Chem., 114, 1 (2005) [Link] 

Structural and Thermodynamic Characterization of Aminotransferases
and Metal Ion Binding Proteins (2006-2010)
 Collaborators
 
  
University of Turku and Åbo Akademi University, Finland
Dr. Anastassios Papageogiou
Turku Center for Biotechnology
 Funding
 		    University of Turku and NCSR “Demokritos”

Publications

› T. Haikarainen et al.,“Structural and thermodynamic characterization of metal ion binding in Streptococcus suis Dpr”, J. Mol. Biol., 405, 448 (2011) [Link]

› E. G. Kapetaniou et al., “Effect of pH on the structure and stability of Bacillus circulans ssp. alkalophilus phosphoserine aminotransferase: Thermodynamic and crystallographic studies”, Proteins, 63, 742 (2006) [Link]


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